IDT
IDT
*2019년 이후 취급 하지 않음BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells
Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:
BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 2 × 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight® Cloning Kits.
E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)
E. cloni 10G BAC-Optimized: F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR) MA052-BAC-Optimized-Replicator.pdf (207,246kb) MA030-E-cloni-10-G-BAC-Optimized-Electro.pdf (209,031kb) MSDS.pdf (118,131kb) |
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