BAC-Optimized Replicator v2.0 & 10G BAC-Optimized Electrocompetent Cells

*2019

BAC-Optimized Replicator v2.0 & 10G BAC-Optimized Electrocompetent Cells

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.
Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:
 
BAC-Optimized Replicator v2.0 Electrocompetent Cells (> 2 1010 cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART BAC or pEZ BAC vectors in the CopyRight Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigens CopyRight Cloning Kits.
 
E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 107 cfu/µg 150 kb BAC DNA; > 1 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.
 
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
E. cloni Cell Lines
Transformation Efficiency
(cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
Blue/White Screening
10G BAC-Optimized Electrocompetent
>1 1010
YES
YES
YES
without IPTG induction
BAC-Optimized Replicator v2.0 Electrocompetent
>2 1010
YES
YES
YES
IPTG induction required
 
 
Replicator v2.0: F- mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara,leu)7697 galUgalrpsnupG (attaraC-PBAD-trfA250 bla attR) -. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)
 
E. cloni 10G BAC-Optimized: F - pro A+B+ lacIqZM15::Tn10 (TetR)] /mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara, leu)7697 galgalrpsnupG - tonA (StrR)
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