Amintra Nickel Rapid Flow Resin

Amintra Nickel Rapid Flow Resin

Amintra Nickel Rapid Flow Resin.jpg

Amintra NiHIS and CoHIS rapid flow resins are affinity resins precharged with Nickel or Cobalt designed for simple and rapid purification of recombinant HIS-tagged protein from cell lysate under denaturing or native conditions. Amintra NiHIS and CoHIS resin consists of 45-165 m agarose beads covalently coupled to a chelating group.

HIS-TAGGED Proteins: Nickel Rapid Flow
Crude extract (lane 2) containing his-tagged fucolose-1-aldolase was batch purified using Amintra NiHIS Rapid Flow (lane 3) and a competitor resins (lane 4,5)). Amintra resin provides high purity and yield.


Supporting matrix
Covalently coupled to agarose resin
Charged metal ion Ni2+ or Co2+
Bead size range
45-165 m
Recommended working pH pH 2.0-12.0
Typical binding capacity ~10 mg HIS-tagged recombinant protein/ml resin
Maximum Flow rate 25 ml/min
Optimum Flow Rate 1 ml/min
Maximum pressure 3 bar
Chemical stability High
Solubility in water Insoluble

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