BigEasy-TSA Electrocompetent Cells

*2019

BigEasy-TSA Electrocompetent Cells

  • Cells required for transformation and propagation of pJAZZ Vectors.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.
BigEasy-TSA Electrocompetent Cells (>2 1010 cfu/µg pKanR DNA) are for use with Lucigens pJAZZ-OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System. The TSA Cells have the telN gene required for high efficiency transformation, and the antA gene required for copy number control with the pJAZZ vector, integrated into the host genome. These cells also give high yield and high quality plasmid DNA due to the endA1 and recA1 mutations. They contain the mcr and mrr mutations, allowing methylated genomic DNA that has been isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.
 
Table 1. E. cloni Competent Cells for BAC, Large or Difficult Fragment Cloning
E. cloniCell Lines
Transformation Efficiency
(cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
Blue/White Screening
BigEasy-TSA Electrocompetent
>2 1010
YES
No
YES
IPTG induction required
BigEasy-TSA: F- mcrA (mrr-hsdRMS-mcrBC) 80dlacZM15 lacX74 endA1 recA1araD139 (ara,leu)7697 galU galK rpsL nupG - tonA AmpR sopAB telN antA
BigEasy-TSA: F- mcrA (mrr-hsdRMS-mcrBC) 80dlacZM15 lacX74 endA1 recA1araD139 (ara,leu)7697 galU galK rpsL nupG - tonA AmpR sopAB telN antA
 
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