Amintra Glutatione Resin

Amintra Glutatione Resin

Amintra Glutatione Resin.jpg
 
GST-TAGGED proteins - Loose Resin
Amintra's Glutathione resin is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to a high crosslinked agarose matrix that provides a high chemical and physical stability and excellent flow rates. The resin is made in the particle size range of 40-165 m and has a dynamic binding capacity of 10 mg recombinant GST/ml resin. Amintra Glutathione is stable with all commonly used buffers and reagents including 0.1M NaOH and organic solvents. Removal of the GST tag can be performed whilst the fusion protein is bound to the column or in solution after elution.
 

Specification:

 
Supporting matrix 4% highly crosslinked agarose resin
Bead size range 40 -165 m (90 micron average)
Recommended working pH pH 4.0-10.0
Typical binding capacity ~10 mg GST-tagged protein/ml resin
Recommended Flow rate 1-2 ml/min/cm2
Maximum Flow rate 4 ml/min/cm2
Maximum pressure 3 bar
Chemical stability High
Solubility in water: Insolubl


 
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