Prepacked Columns

Prepacked Columns

Amintra Protein A columns are packed with Fine Rapid Flow (FRF) beads covalently modified with recombinant Protein A expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with high capacity and affinity for antibodies with typically binding capacity of 25 mg IgG / ml resin.

Prepacked Columns

Amintra Protein A columns are packed with Fine Rapid Flow (FRF) beads covalently modified with recombinant Protein A expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with high capacity and affinity for antibodies with typically binding capacity of 25 mg IgG / ml resin.

The binding chemistry results in fast binding kinetics and low leakage levels delivering high performance and efficient purification and fractionation of IgG subclasses.

Specifications

Bead	Fine Rapid Flow (6% agarose - 35 micron)
Ligand	Recombinant Protein A expressed in E. coli deficient in albumin binding domain
Ligand density	~3 mg Protein A/ml resin
Toxins	Free of Staphyloccocus enterotoxins and hemolysins
Binding capacity	Upto 25 mg Human IgG /ml resin
Recommended Flow rate	1ml/min for 1 ml column 5ml/min for 5 ml column
Max Pressure	3 bar
Application	Automated Liquid chromatography (MPLC, FPLC, AKTA), syringe, peristaltic pump
Connectivity	Standard 10-32 fitting without additional connectors
Storage	4-8 C
pH Stability	3-9 (long term), 2-10 (short term) (protein ligands may hydrolyze at very low pH!

The binding chemistry results in fast binding kinetics and low leakage levels delivering high performance and efficient purification and fractionation of IgG subclasses.

Specifications

Bead	Fine Rapid Flow (6% agarose - 35 micron)
Ligand	Recombinant Protein A expressed in E. coli deficient in albumin binding domain
Ligand density	~3 mg Protein A/ml resin
Toxins	Free of Staphyloccocus enterotoxins and hemolysins
Binding capacity	Upto 25 mg Human IgG /ml resin
Recommended Flow rate	1ml/min for 1 ml column 5ml/min for 5 ml column
Max Pressure	3 bar
Application	Automated Liquid chromatography (MPLC, FPLC, AKTA), syringe, peristaltic pump
Connectivity	Standard 10-32 fitting without additional connectors
Storage	4-8 C
pH Stability	3-9 (long term), 2-10 (short term) (protein ligands may hydrolyze at very low pH!