Amintra Desalting Spin Column

Cross-linked neutral particles with very small pores are packed into spin tubes for rapid desalting and / or buffer exchange. Amintra Desalting Spin Column achieves desalting or buffer exchange in just a few minutes. Upto24 samples (depending on the type of microcentrifuge) of 10 – 100 µl can be processed in one spin. It is a much faster and more efficient approach when compared to dialysis tubes or membrane ultrafiltration.

The small porous particles provide huge surface area with very short diffusion distance, which means small molecules such as salt can be partitioned rapidly. In comparison, both dialysis tube and membrane ultrafiltration have very low surface areas. It always takes much longer to conduct dialysis (typically from a few hours to a few days). Membrane ultrafiltration of small samples always experiences membrane blockage and severe loss of valuable materials

Key benefits:

Rapid desalting or buffer exchange
Very little loss of target molecules (typically > 95% recovery)
Suitable for proteins (> 6,000 dalton) and DNAs (> 10 bp)
DNAse free

Amintra Desalting Spin Column is particularly useful for the following applications:

Desalting of histidine-tagged proteins (e.g. imidazole and NaCl) recovered from IMAC Amintra Desalting Spin Colum
Desalting of samples before loading to SDS-PAGE or GELFREE 8100
Desalting of samples before conducting other analysis
Buffer exchange, for example, after low pH elution
Desalting of DNAs

Amintra_Desalting_Spin_Column.pdf (436,646kb)