TEV-Express

TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can be used in a buffer most suitable for the target protein. TEV-Express is a 52 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins

1 Unit of TEV-Express cleaves >85% of 3 g of control substrate in 1 hour at 30C.

Contents: 1,000 Units in 50 l

Contents: 10,000 Units in 500 l

TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can be used in a buffer most suitable for the target protein. TEV-Express is a 52 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins.

A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TEV-Express or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4C for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors' TEV Protease product.

TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can be used in a buffer most suitable for the target protein. TEV-Express is a 52 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins.

A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TEV-Express or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4C for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors' TEV Protease product.