3C-Express

3C-Express

3C-Express.jpg

 
3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly. 3C-Express has robust activity at 4C and high specific activity. 3C-Express is a 47 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins.

1 Unit of 3C-Express cleaves >95% of 100 mg of target protein at 4C for 16 hours.

Contains: 500 Units in 250 l
Contains: 5,000 Units in 2,500 l
 
A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with 3C Express (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 42 kDa and 26 kDa.
A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with 3C Express (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 42 kDa and 26 kDa.
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