*2019년 이후 취급하지 않음
VersaWave Spectrophotometer
The VersaWave spectrophotometer is the perfect tool for fast and accurate measurement of a wide variety of biomolecules such as protein, DNA and RNA. The VersaWave reads samples in milliseconds and can be used for micro-volume measurements with sample volumes as low as to 0.6 µl. Equally the device can be used as a conventional spectrophotometer. Furthermore the VersaWave extends its capabilities into other areas such as remote sample measurement and on-line ‘real time’ sampling. The VersaWave with its small footprint will suit any laboratory looking for fast, reliable and accurate measurements.
Supplied with control and data acquisition software and fiber optic couplings
Features & Benefits
- Easyto use: preprogrammed applications for DNA, RNA and protein quantitation
- Wide dynamic range up to 12,000 ng/µl DS-DNA: no need to dilute samples
- Highly sensitive and accurate down 3 ng/µl DS-DNA
- Low sample volume required down to 0.6 µl
- Sample can be fully recovered using VersaTip
- Multitude of sample read out options
- Pipette tip
- Droplet cuvettes
- Conventional cuvette
- Flow cells
- No moving parts: virtually maintenance free; no calibration orafter sales service required
DNA/RNA Concentration and Purity
The measurement of absorbance values at 230, 260, 280 and 320nm and their use with standard conversion factors for nucleic acid quantification and purity check is well documented. VersaWave II has stored methods for quantitation of ssDNA, dsDNA and RNA. A cuvette holder accessory is available so that VersaWave can also be used with cuvettes instead of pipette tips accommodating a range of pathlengths: 10mm, 5mm, 1mm, 0.5mm, 0.2mm and 0.125mm.
Proteins
The VersaWave has stored methods for both direct (UV280,IgG, Lysozyme, BSA) and indirect (Bradford, Lowry) protein determinations, and these protocols can be modified to suit local needs as there are many variants. Other methods can be entered and stored as required, and users can construct fully editable calibration curves using from 1 to 50 standards with up to 5 replicates of each, apply polynomial curve fitting, automatically compensate for the path length of the pipette tip or cuvette, store calibration results in a secure project folder and use a blank or blank and high standard in routine operation, or verify all points as required.
Microarray
The labeling efficiency of reactions for microarray measurements can be determined by using the approximate ratio of bases to dye molecules; this can be determined from the absorbance of the nucleic acid at 260 nm and the absorbance of the dye at its max. VersaWave has stored methods for the common dyes while others can easily be added. There is automatic correction for pathlength and cross interference from the dye to ensure that results are both accurate and reliable.
Cell Culture
The measurement of cell culture optical density at 600nm (OD600) is used as a convenient method of estimating cell growth rates and their readiness for harvesting. The VersaWave II offers quick and accurate measurements of OD600 as well as allowing more detailed calibration against standards of known cell populations.
Technical Specification
| Pathlength |
from 40mm to 0.125 mm |
| Sample volume |
as low as 0.6 ul |
| Light Source |
Pulsed xenon lamp (powered for reading only) |
| Lamp Life |
Up to 10 years in normal operation |
| Detector |
3648 pixel CCDArray (UV enhanced) |
| Photometric Linearity |
Better than 1% |
| Photometric Range |
-0.2 to 2.5A |
| Wavelength Accuracy |
190 to 1050nm |
| Spectral Bandwidth |
<2nm |
| Absorbance Precision |
0.003A |
| ReadTime |
2 seconds (includes full wavelength scan) |
| PC communication |
Ethernet |
| Power |
24DC from supplied adapter (100 – 240V) |
| Dimensions |
18 x 18 x 17cm |
| Weight |
3.5kg |
| Software Compatibility |
Professional versions of Windows 7 (32 bit and 64 bit), Windows Vista (32 bit), Windows XP (32 bit) |
| Warranty |
1Year |
IDT
