IMMOLASE¢â is a heat-activated, thermostable DNA polymerase. IMMOLASE provides high yield and improved specificity when compared to standard polymerases and can eliminate the presence of non-specific binding, such as primer-dimers and mis-primed products.
Features & Benefits• Heat-activated ³»¿¼ºÀÇ DNA polymerase• ¶Ù¾î³ª°í °ß°íÇÑ performance • ¾çÀûÀÎ assay ¼öÇà ½Ã ÈǸ¢ÇÑ yield º¸ÀÓ • »ó¿Â¿¡¼µµ ½ÇÇè ÇÒ ¼ö ÀÖ´Â Æí¸®¼º • TA cloning °¡´É ('A' overhang) Applications• Multiplex PCR reaction ƯÈ÷ ³ôÀº ƯÀ̼º
• TA cloning • ¾ÆÁÖ ÀûÀº ¾çÀÇ template • Multiple PCR reaction °¡´É DescriptionIMMOLASE¢â ´Â heat-activated ÇÑ ³»¿¼º DNA polymeraseÀÔ´Ï´Ù. ³ôÀº ¼öÀ²ÀÇ »ê¹°À» Á¦°øÇϸç (Fig.1) ÀÏ¹Ý polymerase¿Í ºñ±³ÇßÀ» ¶§ °¨µµ¸é¿¡¼ ¿ùµîÈ÷ ¶Ù¾î³³´Ï´Ù. ±×¸®°í primer-dimer³ª miss-primed °á°ú¹° µî ºñƯÀÌÀûÀÎ °áÇÕÀ» ÇÏÁö ¾Êµµ·Ï Çϴ Ư¡À» °¡Áö°í ÀÖ½À´Ï´Ù. º¸Åë »ó¿Â¿¡¼´Â enzymeÀÇ È°¼ºÀÌ ¾ø±â ¶§¹®¿¡ PCR ½ÃÀÛ Àü pre-heating °úÁ¤À» 10ºÐ°£ ÁøÇàÀ» ÇØ¾ß ÇÕ´Ï´Ù. ÀÌ pre-heating °úÁ¤À» ÁøÇàÇÏ¸ç ½ÇÇèÀÚ´Â PCR reagentsµéÀ» ¼¯´Â °úÁ¤À» µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö Àֱ⠶§¹®¿¡ setup ½Ã°£À» µû·Î ÀâÁö ¾Ê¾Æµµ µÇ¾î Æí¸®ÇÕ´Ï´Ù. °Ô´Ù°¡ ±âÁ¸ÀÇ ´Ù¸¥ ³»¿¼º DNA polymeraseÀÇ protocolÀ» °¡Áö°í IMMOLASE¢â·Î ½ÇÇè °¡´ÉÇϱ⠶§¹®¿¡ Á¶°ÇÀ» µû·Î ÀâÀ» ¹ø°Å·Î¿òÀÌ ÁÙ¾îµì´Ï´Ù. ¶ÇÇÑ 2X PolyMate Additive(BIO-37041)¸¦ Ãß°¡·Î »ç¿ëÇÏ¿© IMMOLASE¢âÀÇ °¨µµ¿Í ¼º´ÉÀ» ¶Ç Çѹø ³ôÀÏ ¼ö ÀÖ½À´Ï´Ù. ƯÈ÷GC- ³ª AT-°¡ ¸¹Àº DNA sequence³ª secondary structure°¡ ¸¹Àº template¿¡ ¾ÆÁÖ È¿°úÀûÀÔ´Ï´Ù. NotesImmolase is a trademark of Bioline.Components
Concentration5u/¥ìl Storage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsShipped on Dry Ice or Blue Ice. Fact sheet [immolase_dna_polymerase_factsheet.pdf] (360,622kb) protocol [pi_50108_immolase_dna_polymerase_protocol.pdf] (278,499kb) |
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