ImmoMix¢â
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-25020ImmoMix, 2x500 RXNSat -20¡ÆC-·Î±×ÀÎ
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 ImmoMix¢â is a complete ready-to-use high yield heat-activated 2x reaction-mix, which simply requires the user to add water, template and primers, and then pre-heat to 95¡ÆC for 10 minutes to carry out successful PCR assays.




 

Features & Benefits

• Heat-activated ³»¿­¼ºÀÇ DNA polymerase
• ¶Ù¾î³ª°í °ß°íÇÑ performance
• ¾çÀûÀÎ assay ¼öÇà ½Ã ÈǸ¢ÇÑ yield º¸ÀÓ
• »ó¿Â¿¡¼­µµ ½ÇÇè ÇÒ ¼ö ÀÖ´Â Æí¸®¼º
• TA cloning °¡´É ('A' overhang)
 

Applications

• Multiplex PCR reaction ƯÈ÷ ³ôÀº ƯÀ̼º
• TA cloning
• ¾ÆÁÖ ÀûÀº ¾çÀÇ template
• Multiple PCR reaction °¡´É

 

Description

IMMOMIX¢â ´Â heat-activated ÇÑ ³»¿­¼º DNA polymerase ÀÔ´Ï´Ù. ³ôÀº ¼öÀ²ÀÇ »ê¹°À» Á¦°øÇϸç ÀÏ¹Ý polymerase¿Í ºñ±³ÇßÀ» ¶§ °¨µµ¸é¿¡¼­ ¿ùµîÈ÷ ¶Ù¾î³³´Ï´Ù. ±×¸®°í primer-dimer³ª miss-primed °á°ú¹° µî ºñƯÀÌÀûÀÎ °áÇÕÀ» ÇÏÁö ¾Êµµ·Ï Çϴ Ư¡À» °¡Áö°í ÀÖ½À´Ï´Ù. º¸Åë »ó¿Â¿¡¼­´Â enzymeÀÇ È°¼º ÀÌ ¾ø±â ¶§¹®¿¡ PCR ½ÃÀÛ Àü pre-heating °úÁ¤À» 10ºÐ°£ ÁøÇàÀ» ÇØ¾ß ÇÕ´Ï´Ù. ÀÌ pre-heating °úÁ¤À» ÁøÇàÇÏ¸ç ½ÇÇèÀÚ´Â PCR reagentsµéÀ» ¼¯´Â °úÁ¤À» µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö Àֱ⠶§¹®¿¡ setup ½Ã°£À» µû·Î ÀâÁö ¾Ê¾Æµµ µÇ¾î Æí¸®ÇÕ´Ï´Ù. °Ô´Ù°¡ ±âÁ¸ÀÇ ´Ù¸¥ ³»¿­¼º DNA polymeraseÀÇ protocolÀ» °¡Áö°í IMMOMIX¢â·Î ½ÇÇè °¡´ÉÇϱ⠶§¹®¿¡ Á¶°ÇÀ» µû·Î ÀâÀ» ¹ø°Å·Î¿òÀÌ ÁÙ¾îµì´Ï´Ù. ¶ÇÇÑ 2X PolyMate Additive(BIO-37041)¸¦ Ãß°¡·Î »ç¿ëÇÏ¿© IMMOMIX¢âÀÇ °¨µµ¿Í ¼º´ÉÀ» ¶Ç Çѹø ³ôÀÏ ¼ö ÀÖ½À´Ï´Ù. ƯÈ÷ GC- ³ª AT-°¡ ¸¹Àº DNA sequence³ª secondary structure°¡ ¸¹Àº template¿¡ ¾ÆÁÖ È¿°úÀûÀÔ´Ï´Ù.

Notes

ImmoMix is a trademark of Bioline.

Components

Reagent
100 Reactions
500 Reactions
ImmoMix
2 x 1.25ml
10 x 1.25 ml
50mM MgCl2 Solution
1.2ml
1.2ml

Volume

BIO-25019: 100 x 50¥ìl Reactions: 2 x 1.25ml
BIO-25020: 500 x 50¥ìl Reactions: 10 x 1.25ml

Concentration

2x

Storage & Stability

All components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.
Components may also be stored at +4¡ÆC if required, although storage at -20¡ÆC is recommended. When stored at +4¡ÆC, reagents will remain stable for a period of two weeks from date of receipt.

Shipping conditions

On Dry Ice or Blue Ice.
protocol [pi_50109_immomix_v2.pdf] (325,401kb)
Fact sheet [immolase_dna_polymerase_factsheet.pdf] (360,622kb)
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