MyTaq¢â HS Red DNA Polymerase
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-21115MyTaq HS Red DNA Polymerase1000 UNITSat -20¡ÆC-·Î±×ÀÎ
BIO-21116MyTaq HS Red DNA Polymerase2500 UNITSat -20¡ÆC-·Î±×ÀÎ
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 MyTaq¢â HS Red DNA Polymerase is a, red-colored, high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. MyTaq HS Red does not exhibit polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation.
 




Features & Benefits

Extremely sensitive - delivering very high yields 
Highest perfromance and specificity - resulting from proprietary buffer system and MyTaq enzyme 
Ready to use - red dye and formulation for direct gel loading 
Fast activation times - powered by new generation antibody-based hot-start polymerase 
Flexible - fast PCR reactions or standard both possible

Applications

High-throughput PCR 
Assays with prolonged reaction setup on the bench or liquid handling 
Amplification of challenging targets susceptible to mispriming 
Colony PCR 
Multiplexing 
Specific amplification of difficult templates (GC rich) 
Genotyping 
TA cloning 
Two-Step RT-PCR

Description

MyTaq¢â HS Red DNA Polymerase is a high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR.MyTaq HS Red does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation.
The advanced formulation of MyTaq HS Red allows fast cycling conditions (fig. 1), considerably reducing the reaction time without compromising PCR specificity or yield (fig. 2). This new enzyme from Bioline is supplied with 5x MyTaq buffer system, a proprietary formulation that saves time and delivers superior results, as it contains dNTPs, MgCl2 and enhancers at optimal concentrations, thereby removing the need for further optimization.
The specially designed MyTaq Red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after PCR without the need to add loading buffer. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).
MyTaq HS Red only requires only the addition of template, primers and water and so reduces the risk of pipetting errors and contamination, as well the time needed to set-up reactions.

Notes

MyTaq is a trademark of Bioline.

Components

Reagent
250 Units
1000 Units
2500 Units
MyTaq HS Red DNA Polymerase
1 x 50¥ìl
1 x 200¥ìl
2 x 250¥ìl
5x MyTaq Reaction Buffer
2 x 1ml
8 x 1ml
14 x 1.5ml

Concentration

5u/¥ìl

Storage & Stability

All components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

On Dry Ice or Blue Ice.
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