MyTaq¢â HS Red DNA Polymerase is a, red-colored, high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. MyTaq HS Red does not exhibit polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation.
Features & BenefitsExtremely sensitive - delivering very high yieldsHighest perfromance and specificity - resulting from proprietary buffer system and MyTaq enzyme
Ready to use - red dye and formulation for direct gel loading
Fast activation times - powered by new generation antibody-based hot-start polymerase
Flexible - fast PCR reactions or standard both possible ApplicationsHigh-throughput PCRAssays with prolonged reaction setup on the bench or liquid handling
Amplification of challenging targets susceptible to mispriming
Colony PCR
Multiplexing
Specific amplification of difficult templates (GC rich)
Genotyping
TA cloning
Two-Step RT-PCR DescriptionMyTaq¢â HS Red DNA Polymerase is a high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR.MyTaq HS Red does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation.
The advanced formulation of MyTaq HS Red allows fast cycling conditions (fig. 1), considerably reducing the reaction time without compromising PCR specificity or yield (fig. 2). This new enzyme from Bioline is supplied with 5x MyTaq buffer system, a proprietary formulation that saves time and delivers superior results, as it contains dNTPs, MgCl2 and enhancers at optimal concentrations, thereby removing the need for further optimization.
The specially designed MyTaq Red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after PCR without the need to add loading buffer. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).
MyTaq HS Red only requires only the addition of template, primers and water and so reduces the risk of pipetting errors and contamination, as well the time needed to set-up reactions. NotesMyTaq is a trademark of Bioline. Components
Concentration5u/¥ìl Storage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsOn Dry Ice or Blue Ice. protocol [pi_50153_mytaq_hs_red_dna_polymerase_v2.pdf] (402,680kb) Fact sheet [MyTaq¢â_HS_DNA_Polymerase_productsheet.pdf] (624,501kb) |
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