MyTaq¢â HS Red Mix is a ready-to-use, direct gel load, red colored, 2x mix for fast, highly-specific hot-start PCR.
MyTaq HS Red Mix is powered by antibody mediated hot-start and does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield. Features & Benefits• »õ·Ó°Ô °³¹ßµÈ antibody ±â¹ÝÀÇ hot-start polymerase
• ³ôÀº specificity ¿Í ¿ì¼öÇÑ performance • »õ·Î¿î buffer : ultra-pure dNTPs ¿Í MgCl2 • ¹Ù·Î gel loadingÀÌ °¡´ÉÇÏ°Ô red-dye Æ÷ÇÔ °¡´É • °£ÆíÇÑ all-in-one mastermix ApplicationsHigh-throughput PCR Assays with prolonged reaction setup on the bench or liquid handlingAmplification of challenging targets susceptible to mispriming
Multiplexing
Colony PCR
Specific amplification of difficult templates (GC rich)
Genotyping
TA cloning
Two-Step RT-PCR
Instrument CompatibilityCompatible with Fast and Standard PCR instruments.
DescriptionMyTaq¢â HS Mix´Â Complex genomic DNA¿Í °° Àº ¾î·Á¿î template¸¦ º¸´Ù ½±°í Á¤È®ÇÏ°Ô ÁõÆøÇϱâ À§ÇØ Bioline¿¡¼ »õ·Ó°Ô °³¹ßÇÑ antibody ±â¹ÝÀÇ hot start PCR enzyme ÀÔ´Ï´Ù. ¼öÀ², °¨µµ, ¼Óµµ¸¦ ÁÁ°Ô ÇÒ ¼ö ÀÖµµ·Ï ÃֽŠ±â¼úÀ» ÀÌ¿ëÇÏ¿© PCR enzyme°ú DNA¿ÍÀÇ Ä£È·ÂÀ» ³ô¿©ÁÖ¾ú½À´Ï´Ù. »ó¿Â¿¡¼ reaction mix¸¦ ¸¸µé ¼ö ÀÖÀ¸¹Ç·Î ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ ÀÌ mastermix¿¡´Â dNTPs, MgCl2, enhancerµéÀÌ ÃÖÀûÀÇ ³óµµ·Î µé¾î°¡ ÀÖ¾î¼ optimization ¾øÀ̵µ ¿ì¼öÇÑ °á°ú¸¦ ¸¸µé ¼ö ÀÖ½À´Ï´Ù. ÀÌ¹Ì ¸ðµç ÇÊ¿ä½Ã¾àÀÌ ¼¯ÀÎ MyTaq¢â HS Mix´Â ready-to-use 2x mix typeÀ¸·Î ºü¸¥ °á°ú¸¦ °¡Á®¿Ã ¼ö ÀÖ´Â °í°¨µµÀÇ PCR enzymeÀÔ´Ï´Ù. ÀÌ Á¦Ç°Àº ºü¸¥ cycling conditionÀ¸·Î ÁøÇà ÇÒ ¼ö ÀÖµµ·Ï setting µÇ¾î °¨µµ³ª ¼öÀ²Àº ³ôÀ¸¸é¼ ½ÇÇè ½Ã°£Àº ´ÜÃà ½Ãų ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ red dye¸¦ Ãß°¡·Î Æ÷ÇÔÇÏ°í ÀÖ¾î PCR ½ÇÇèÀÌ ³¡³ª°í È®ÀÎ ÀÛ¾÷ ½Ã µû·Î sample buffer¸¦ ³Ö¾îÁÙ ÇÊ¿ä ¾øÀÌ ¹Ù·Î agarose gel loadingÀÌ °¡´ÉÇÏ¿© º¸´Ù Æí¸®ÇÕ´Ï´Ù.
Components
Concentration2xStorage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsOn Dry Ice or Blue Ice. protocol [pi_50154_mytaq_hs_red_mix_v2.pdf] (403,060kb) Fact sheet [MyTaq¢â_HS_DNA_Polymerase_productsheet.pdf] (624,501kb) |
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