The New MyTaq¢â Blood-PCR Kit offers very fast, highly-specific, direct PCR from a wide range of human and animal whole blood samples, including those preserved with anticoagulants.
Features & Benefits¡Ü Extraction Free – º¹ÀâÇÑ blood DNA extraction °úÁ¤ÀÌ ÇÊ¿ä ¾øÀ½
¡Ü µ¶Æ¯ÇÑ Buffer systemÀ¸·Î ³ôÀº °¨µµ¿Í PCR ¼º°ø·üÀ» º¸ÀåÇÔ
¡Ü Versatile – ¿©·¯speciesÀÇ blood sample·Î ½ÇÇè °¡´É
¡Ü °í°¨µµ¸¦ ÀÚ¶ûÇÏ´Â MyTaq HS polymerase Æ÷ÇÔ
¡Ü Red dye°¡ Æ÷ÇԵǾî ÀÖÀ½
Applications•Animal, human blood sample »ç¿ë °¡´É(EDTA, citrate, heparin Æ÷ÇԵǾî À־ »ç¿ë °¡´É)
•Multiplexing
•GC rich template
•Longer amplicons •Animal, human blood sample »ç¿ë °¡´É(EDTA, citrate, heparin Æ÷ÇԵǾî À־ »ç¿ë °¡´É)
•Multiplexing
•GC rich template
•Longer amplicons
DescriptionMyTaq¢â Blood-PCR KitÀº ¸Å¿ì ºü¸£°í Á¤È®ÇÑ PCRÀ» ¿©·¯ Á¾ÀÇ (human ¹× animalÀ» Æ÷ÇÔ) blood sampleµéÀ» »ç¿ëÇÏ¿© °¡´ÉÇÏ°Ô ÇÏ´Â mastermix ŸÀÔÀÇ kit ÀÔ´Ï´Ù. ÇØ´ç Á¦Ç°¿¡ µé¾îÀÖ´Â buffer´Â ´õÀÌ»óÀÇ º¹ÀâÇÑ ÃßÃâ°úÁ¤ ȤÀº Á¤Á¦°úÁ¤ÀÌ ÇÊ¿ä ¾ø°í µû·Î ÷°¡¹°À» ´õ ³ÖÀ» ÇÊ¿äµµ ¾ø¾î ¸Å¿ì °£ÆíÇÕ´Ï´Ù. Ưº°È÷ °³¹ßµÈ MyTaq¢â Blood-PCR kitÀ̱⠶§¹®¿¡ ºü¸¥ cycling À¸·Î ½ÇÇèÀ» ³¡³¾ ¼ö ÀÖÀ¸¸é¼ yield³ª °¨µµ ¸é¿¡¼´Â ¿©ÀüÈ÷ ¶Ù¾î³ °á°ú¸¦ Á¦°øÇÕ´Ï´Ù.
º¸Åë Ç÷¾× ³»¿¡ Á¸ÀçÇÒ ¼ö ÀÖ´Â Ç×ÀÀ°íÁ¦ - EDTA, citrate, heparin- ´Â PCRÀ» Çϴµ¥ ÀÖ¾î ¹æÇØµÉ ¸¸ÇÑ ¹°ÁúÀ̱⠶§¹®¿¡ ¹Ì¸® Á¦°ÅÇÏ°í PCR ½ÇÇèÀ» ÇÏ´Â°Ô º¸ÅëÀ̾úÁö¸¸ MyTaq¢â Blood-PCR Kit¸¦ »ç¿ëÇÔÀ¸·Î½á ÇØ´ç PCR ÀúÇØÁ¦µéÀÌ Ã·°¡µÇ¾î À־ ¹«¸®¾øÀÌ PCR ½ÇÇèÀÌ °¡´ÉÇÕ´Ï´Ù. ¶ÇÇÑ ±æÀÌ°¡ ±ä amplicons³ª GC ÇÔ·®ÀÌ ³ôÀº template¸¦ targetÀ¸·Î PCRÀ» Çϼŵµ MyTaq¢â Blood-PCR Kit¸¦ »ç¿ëÇÏ½Å´Ù¸é º° ¹«¸®¾øÀÌ ¼º°øÀûÀÎ PCR °á°ú¹°À» È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
humanÀ̳ª animalÀÇ whole blood sampleÀ» °¡Áö°í PCRÀ» ÇÏ´õ¶óµµ PCR »ê¹°Àº ¼º°øÀûÀ¸·Î ¾òÀ¸½Ç ¼ö ÀÖ½À´Ï´Ù.
±×¸®°í Fast condition¿¡¼ PCRÀÌ °¡´ÉÇÏ°í °í°¨µµÀÇ polymerase°¡ Æ÷ÇԵǾî Àֱ⠶§¹®¿¡ multiplex PCR¿ë°ú high-throughput genotyping assay¿ëÀ¸·Îµµ ¸Å¿ì ÀûÇÕÇÑ Á¦Ç°ÀÔ´Ï´Ù. NotesMyTaq is a trademark of Bioline. Components
Concentration2x Storage & StabilityMyTaq Blood-PCR Kit can be stored at -20¡ÆC. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label. Shipping conditionsOn dry or blue ice. Fact sheet [MyTaq¢â_Blood-PCR_Kit_fact_sheet.pdf] (376,931kb) protocol [MyTaq¢â_Blood-PCR_Kit_protocol.pdf] (351,433kb) |
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