Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from
uracil-containing single-stranded or double-stranded DNA, but not from RNA or
oligonucleotides.
Features & BenefitsRemoves uracils fron DNA - causes fragmentation of DNA and stops amplification in a PCREasy-to-use - novel, sensitive mutant is irreversibly
inactive after heating ApplicationsControl of carry-over contamination in PCRAs a probe for protein-DNA interaction studies
Cloning of PCR products DescriptionUracil DNA Glycosylase (UDG) catalyzes the release of uracil from
uracil-containing single-stranded or double-stranded DNA, but not from RNA or
oligonucleotides (6 or fewer bases).
UDG is active over a broad pH range with an optimum at pH 8.0, does not
require a divalent cation, and is inhibited by high ionic strength
(>200mM).
Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases,
exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene
of Uracil DNA Glycosylase from E. coli.
Components
Concentration1u/¥ìl Storage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability.
Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full
activity of the reagents is retained until the expiry date indicated on the
outer box label. pi_50265_uracil_dna_glycosylase_udgase_v2.pdf (339,353kb) |
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