"I assessed BIOTAQ for amplicon sequencing and found a high level of (observable) reproducibility."

- British Antarctic Survey, Cambridge

Consistent results Premium Taq polymerase, suited to a wide range of applications Processes fragments of up to 5Kb Leaves ¢¥A¢¥ overhang Available as ready-to-use 2x reaction mixes (BioMix¢â / BioMix¢â Red)

Routine PCR applications TA cloning

BIOTAQ¢â is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5¢¥-3¢¥ exonuclease activity and leaves an ¢¥A¢¥ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

BIOTAQ is supplied with 10x NH₄-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl₂ is provided to allow reaction conditions to be adjusted to suit the template.

The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

BIOTAQ¢â DNA Polymerase is purified from Thermus aquaticus.

BIOTAQ DNA Polymerase 10x NH₄ Reaction Buffer 50mM MgCl₂ Solution

One unit will incorporate 10nmoles of dNTPs in 30min at 72¡ÆC.

5u/¥ìl

BIOTAQ can be stored for 12 months at -20¡ÆC.

Amaral, I.P. & Johnston, I.A. J. Exp. Biol. 214, 2125-2139 (2011). Coutinho, C.P., et al. Infect. Immun. 79, 2950-2960 (2011). Lora, J., et al. PNAS 108, 5461-5465 (2011). Palazon, A., et al. Can. Res. 71, 801-811 (2011). del Hoyo, A. & Pedrola-Monfort, Plant Systemat. Evol. 273(3-4), 151-67 (2008). Lopez-Lluch, G., et al. PNAS 103(6), 1768-73 (2006). Cervero, A., et al. Clin. Endocrinol. Metab. 89, 2442–51 (2004). Brigido, C., et al. Vet. Parasitol. 123(1-2), 17-23 (2004). Knight, J.C., et al. Nat. Gene. 33(4), 469-75 (2003). Goodall-Copestake, W.P., et al. Heredity, 104(5), 513-518. (2010)

BIOTAQ is a trademark of Bioline.