IDT
IDT
 Features & BenefitsSensitivity - allows reproducible detection of low-copy number templates
Reproducibility - generates real-time data reliably and reproducibly
Specific - proprietary hot-start chemistry minimizes non-specific amplification
Broad dynamic range - a dynamic range of up to eight orders of magnitude
Instrument CompatibilityABI 7000, 7300, 7700, 7900, 7900HT, StepOne™ and StepOne™ Plus. (See product selection table). However the kit is also compatible with several instruments that do not require the use of ROX™, such as the Qiagen Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480. DescriptionSensiMix SYBR® Hi-ROX kit is a premixed high performance product designed for superior sensitivity and specificity (Fig. 1) on various real-time instruments in which a ROX signal is required, particularly for those block-based platforms which are more sensitive to the dye levels. Powered by a proprietary highly optimized chemical hot-start PCR enzyme, the mix has been developed to provide the high sensitivity and specificity required for demanding assays or for those assays where template is limited.
SensiMix SYBR® Hi-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. Components
NotesSensiMix is a trademark of Bioline Reagents Ltd. VolumeQT605-05: 500 x 50µl Reactions: 10 x 1.25ml
QT605-20: 2000 x 50µl Reactions: 40 x 1.25ml
Storage & StabilityAll kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label. Shipping conditionsShipped on dry/blue ice. protocol [pi_50231_sensimix_sybr_hi_rox_kit_v1.pdf] (432,692kb) |
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