IDT
IDT|
IDT에서 제공하는 MGB Eclipse® Probes는 ISO 13485 인증이 포함된 GMP 시설에서 합성한 human IVD 전용으로만 사용할 수 있는 probe입니다. PCR은 분자 진단 기술에 있어 매우 중요한 실험 중 하나이며, gold standard qPCR(5’ nuclease assays)은MGB(minor groove binder)기술을 도입한 probe를 사용한 기법입니다. 다양한 형광을 사용하여 multiplex로 디자인을 하기 쉽고 MGB는 hybridization을 안정화하고 Tm값을 올려가 짧은 올리고가 AT-rich region을 detection하거나 allelic discrimination을 하는데 매우 적합합니다.
Specifications
Dye - Quencher Final yield 6nmole 20nmole 50nmole FAM - MGB Eclipse HEX - MGB Eclipse TET- MGB Eclipse YAK - MGB Eclipse Inquire Inquire Inquire  Comparison qPCR studies of multiple MGB™ probe and primer sets manufactured by IDT versus those made by another leading manufacturer demonstrated equivalent genotyping calls for KRAS mutations and high specificity for the appropriate wild-type or mutant allele (Figure 1). End-point fluorescent signal intensities were similar or slightly higher using MGB Eclipse® Probes from IDT.  Figure 1. Genotyping Results From KRAS G12R qPCR Assays Using Probe and Primers Manufactured by IDT and Another Leading Manufacturer. The KRAS G12R qPCR assays used MGB Eclipse® probes (FAM dye—wild-type probe; TET™ dye—mutant probe) and primers made by either IDT or another leading manufacturer (Company L). Reactions (10 µL) were run with 104 copies of wild-type, mutant, or pooled wild-type/mutant template (IDT® gBlocks® Gene Fragments) and TaqMan® Gene Expression Master Mix (Life Technologies) on a CFX384 Touch™ Real-Time PCR Detection System (BioRad). Cycling conditions were 3 min. 95°C; 50 x (10 sec. 95°C, 30 sec. 60°C). |
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