IDT
IDT
TransIT®-2020 Transfection ReagentA high performance, animal-free, broad spectrum DNA transfection reagent
특징
TransIT-2020 시약은 포유 동물 세포에 광범위하게 transfection을 할 수 있는 다목적의 신제품입니다.
이 시약은 폭넓은 호환성이 있도록 동물 구성 요소로 이루어져 있고 주된 경쟁사들의 결과들을 능가하는 결과를 제공해 드립니다.  Figure 1. TransIT®-2020 Reagent Efficiently Transfects Human Induced Pluripotent Stem (iPS) Cells. The TransIT®-2020 Transfection Reagent was used to transfect 0.5 x 106 iPS cells with a ZsGreen expressing plasmid (Clontech). Transfections were performed in 6-well plates using 7.5 µl of TransIT®-2020 Transfection Reagent to deliver 2.5 µg of DNA (3:1, reagent: DNA). Cells were visualized 24 hours post-transfection and imaged at 4X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast, and (B) green fluorescence. Cells were also assayed 24 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows untransfected cells (black line) compared to cells transfected with plasmid using TransIT®-2020 (green line). See more information on stem cell applications.
 Figure 2. TransIT®-2020 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. Human umbilical vein endothelial cells (HUVEC) were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Error bars represent the standard deviation of triplicate wells.
 Figure 3. Superior Gene Expression in a Broad Spectrum of Cell Types. The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1 µg/well) according to industry accepted testing protocols. Reagent to DNA ratios were optimized for each cell type: TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine® 2000 (Life Technologies, 1.5:1, 3:1 or 5:1). Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT®-2020 and presented as a percentage of luciferase expression. FuGENE is a registered trademark of Fugent LLC. Lipofectamine is a trademark of Life Technologies Corporation.
Storage Conditions: Store at -20°C
Product Guarantee: 1 year ml055_transit_2020_transfection_reagent.pdf (363,461kb) |
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