단종안내) Product Discontinuation Notice- DNA Origami ( 2022.04.30)

Scaffold DNAs, Staple Strand Oligos, and Folding Kits for DNA origami self-assembly

DNA origami are intricate, DNA-based nanostructures that are precisely folded into specific shapes via sequence-programmed self-assembly. These nanostructures can be chemically modified, and they can be used for a variety of applications, such as delivering drug molecules or developing novel biosensors and nanodevices.

As leaders in their respective fields, IDT and tilibit nanosystems (Garching, Germany) have partnered to make DNA origami reagents available through a single portal.

This partnership offers you:

Streamlined ordering of DNA origami reagents (Scaffold DNA + Staple Strand Oligos, or Folding Kit + Staple Strand Oligos)
Design guidance and support from the DNA origami experts at tilibit nanosystems
Access to high quality, IDT-manufactured Staple Strand Oligos that are verified by ESI-mass spectrometry, providing complete reagent confidence

DNA origami self-assembly requires 2 types of DNA, a scaffold strand and a mixture of staple strands. A scaffold strand is a long piece of single-stranded DNA. Staple strands are single-stranded 30–80 nt DNA oligos that direct the sequence-specific folding of the scaffold strand into the desired configuration.

The single-stranded Scaffold DNAs are produced by tilibit nanosystems and are available in 3 lengths—7249, 7560, and 8064 bases—either individually or as part of a convenient Folding Kit. The tilibit nanosystems Folding Kits contain Scaffold DNA, Folding Reaction Buffer, and an EDTA-free Loading Dye, custom developed to support straight bands in agarose gel electrophoresis. Staple Strand Oligos are synthesized by IDT.

Because DNA origami design is complex, we recommend that new users contact the tilibit nanosystems DNA origami experts for design assistance prior to ordering DNA origami reagents, including the reagents required to build the predesigned structures described below (Table 1).

Predesigned DNA origami

Tilibit nanosystems offers 6 standard DNA origami structures. See Table 1 and www.tilibit.com/collections/prefabricated-structures for details. Contact tilibit nanosystems to determine whether one of these predesigned structures fits your research needs or whether you will need a custom design. Note: To minimize aggregation, all structures are passivated by including poly-T sequences or single-stranded scaffold loops as described in Table 1.

Table 1. Available DNA origami structures. tilibit nanosystems offers these 6 predesigned structures. See www.tilibit.com/collections/prefabricated-structures for more information.

Custom DNA origami

In addition to offering the above 6 predesigned structures, tilibit nanosystems will work with you to develop custom designs for specific research needs. Contact tilibit nanosystems directly for more information on custom designs.

Ordering

To determine the best DNA origami structure for your unique research needs, we recommend that new users contact the tilibit nanosystems DNA origami experts for design assistance prior to ordering Scaffold DNA, Folding Kits, and Staple Strand Oligos.

Single-stranded Scaffold DNA

Scaffold DNAs (produced by tilibit nanosystems) are available in 3 lengths: 7249 bases (type p7249, identical to M13mp18), 7560 bases (type p7560), and 8064 bases (type p8064). Each length of single-stranded Scaffold DNA is available with a choice of volumes and concentrations to best fit your experimental needs.

Product	Catalog #	Unit Size	Price	Order Link
Scaffold DNA, type p7249 Scale 1	1081306	50 pmol (0.5 mL at 100 nM)	Inquire	Click here to order
Scaffold DNA, type p7249 Scale 2	1081307	200 pmol (2 mL at 100 nM)	Inquire	Click here to order
Scaffold DNA, type p7249 Scale 3	1081308	800 pmol (2 mL at 400 nM)	Inquire	Click here to order
Scaffold DNA, type p7560 Scale 1	1081309	50 pmol (0.5 mL at 100 nM)	Inquire	Click here to order
Scaffold DNA, type p7560 Scale 2	1081310	200 pmol (2 mL at 100 nM)	Inquire	Click here to order
Scaffold DNA, type p7560 Scale 3	1081311	800 pmol (2 mL at 400 nM)	Inquire	Click here to order
Scaffold DNA, type p8064 Scale 1	1081312	50 pmol (0.5 mL at 100 nM)	Inquire	Click here to order
Scaffold DNA, type p8064 Scale 2	1081313	200 pmol (2 mL at 100 nM)	Inquire	Click here to order
Scaffold DNA, type p8064 Scale 3	1081314	800 pmol (2 mL at 400 nM)	Inquire	Click here to order

Folding Kits

Folding Kits (tilibit nanosystems) contain Scaffold DNA (50 pmol; 0.5 mL at 100 nM), 10X Folding Reaction Buffer (500 µL), MgCl2 Stock Solution (500 µL at 200 mM), and 6X tilibit Gel Loading Dye (1000 µL) for gel electrophoretic analysis. Note that the staple strands are custom sequences specific to your DNA origami structure, and need to be ordered separately. See below.

Product	Catalog #	Price	Order Link
Folding Kit Basic, type p7249	1081315	Inquire	Click here to order
Folding Kit Basic, type p7560	1081316	Inquire	Click here to order
Folding Kit Basic, type p8064	1081317